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[KO]Rab5 Rabbit mAb

Western blot analysis of Rab5 in HeLa(WT) and Rab5-Knockout HeLa(KO) using Rab5 antibody.
Western blot analysis of Rab5 in K562, C6, 3T3, Hela lysates using Rab5 antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Rab5 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	P20339
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Purification	Affinity Purified
Calculated MW	24 KDa

Additional Information
Other Names	RAB5A
Dilution	WB~~1:1000-1:5000 IHC-P~~1:50~200
Format	Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis.

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¥ 1,250.00

Cat# AP75987

Size:

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100 µl

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¥ 2,050.00

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[KO]Rab5 Rabbit mAb

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